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KMID : 0900920030270020097
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Korean journal of Animal Reproduction
2003 Volume.27 No. 2 p.97 ~ p.102
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Detection of PSS Gene through Genomic DNA of Umbilical Cord Blood by PCR-RFLP in Piglets
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Kim Gye-Woong
Lee Jong-Wan
Yoo Jae-Young
Park Hong-Yang
Yoon Jong-Man
Cho Gyu-Seok
Kim Geon-Joong
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Abstract
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This study was carried out to find out PSS(Porcine Stress Syndrome) with the PSE(Pale, Soft, Exudative) in different piglets. These experiments were accomplished with the aid of PCR-RFLP(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The samples were collected and examined from umbilical cord blood of piglets of Yorkshire, Landrace and Crossbred. And then, the PCR products were digested by restriction enzyme, Hha I. The results obtained were as follows; The PCR products of the blood genomic DNA of ryanodine receptor gene were length of 1 .8kb in umbilical cord blood. Normal type(NN), heterozygous type(Nn) and recessively homozygous type(nn, PSS) as a result of digestion of restriction enzyme, Hha 1, were 90.0%, 10.0% and 0.0% in Yorkshire piglets, 76.2%, 19.0% and 4.8% in Landrace, 69.1%, 23.8% and 7.1% in crossbred, respectively. As already showing the above results, the blood from piglets umbilical cord can be availably used for the determination of genotypes of PSS because of easiness of blood collection without stress in live piglets.
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KEYWORD
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PSS, Umbilical cord blood, PCR-RFLP, Restriction enzyme
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